Lattice Light-Sheet and Fresnel Incoherent Correlation Holography
Description
Light sheet microscopy is a high-speed, low-excitation intensity, 3D imaging technique. While light sheet microscopy provides advantages over other known microscopy techniques, excitation light sheets disperse rapidly in refractive tissue which limits the penetration depth of a target or sample material. Lattice light-sheet microscopy uses a convergent lattice of Bessel beams to generate the light sheet but is still limited to an imaging depth of approximately 100 μm in live tissue due to optical distortions. This system and method are designed to perform incoherent holographic microscopy imaging to generate a 3D image of a sample.
Additional Information
Patent number and inventors
PCT/US2021/17102
Christopher Mann
Potential applications
Analytical applications
Benefits and advantages
This system utilizes a modified dual-lens Fresnel incoherent correlation holography technique to produce a complex hologram and to provide a reconstruction distance needed for the reconstruction of the hologram. Additionally, this system enables the generation of 3D holographic images without requiring the movement of any of a sample stage or detection microscope objective which simplifies the system setup and reduces the need for mechanical components and controls.
Case number and licensing status
2021-030
This invention is available for licensing.