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  6. Methods and Assays for Subtyping Staphylococcus Aureus Clonal Complex Eight Strains

Methods and Assays for Subtyping Staphylococcus Aureus Clonal Complex Eight Strains

Description

Distinguishing among the sub-lineages of Staphylococcus aureus clonal complex 8 (CC8) is critical for purposes of epidemiology and surveillance, especially as the epidemiologic separation between hospital-acquired (HA) and community-associated (CA) strains disappear. The technology is a new typing scheme for CC8 isolates that is rapid and easy to use. Also, the technology is based on stable genomic markers. The assay uses real-time PCR (polymerase chain reaction) and a labeled probe for detection.

Additional Information

Patent number and inventor

16/607,716

Paul Keim, Elizabeth Driebe, Jolene Bowers, David Engelthaler, Brandi Limbago, James Rasheed, Linda McDougal, and Valerie Albrecht.

Potential applications

Clinical applications to healthcare-acquired infections.

Benefits and advantages

The assay allows for the identification of canonical single nucleotide polymorphisms (canSNPs) from a Staphylococcus aureus CC8 phylogeny to target each of the major lineages. The present invention involves a canSNP-based approach to eliminate the lineage confusion observed with pulse field gel electrophoresis (PFGE), spa typing, and mobile genetic marker typing, as SNPs are inherently stable and quantify relatedness among strains. The assay employs real-time PCR as a rapid, scalable technique that is common in public health and reference laboratories, making the assay an attractive tool for surveillance and epidemiology.

Case number and licensing status

2016-018

This invention is available for licensing.