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  6. Allelic Discrimination Assays for Important Klebsiella Pneumoniae Strains

Allelic Discrimination Assays for Important Klebsiella Pneumoniae Strains

Description

This technology implements a real-time polymerase chain reaction (RT-PCR) assay, targeting single nucleotide polymorphisms (SNPs) specific for ST258 and CG258. With this technology, one or more strains of Klebsiella pneumoniae can be detected by amplifying specific sequences within its genome.

Additional information

Patent number and inventor

10,214,782

Jolene Bowers, Brandon Kitchel, Elizabeth Driebe, Duncan MacCannell, Chandler Roe, Darrin Lemmer, Tom de Man, J. Kamile Rasheed, David Engelthaler, Paul Keim, and Brandi Limbago.

Potential applications

Clinical applications for healthcare-associated infections.

Benefits and advantages

Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae are one of the most widely disseminated carbapenem-resistant Enterobacteriaceae (CRE) pathogens affecting healthcare; KPC is associated with high morbidity and mortality rates. Genomic signatures for both the CG258 and ST258 strains were identified. The technology can detect and distinguish between the two strains from other strains of KPC with 100% sensitivity and specificity. In addition, the technology provides a cost-effective method to screen and survey for the various strains.

Case number and licensing status

2016-002

This is available for licensing.