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  6. Universal Multiplex Indexing Strategy for Next-Generation Amplicon Sequencing (UMINGAS)

Universal Multiplex Indexing Strategy for Next-Generation Amplicon Sequencing (UMINGAS)

Description

The technology relates to systems and methods that can be used in conjunction with sequencing processes and preparing amplicon products for sequencing through the use of a universal tail-based indexing strategy. In addition, the systems and methods allow for the simultaneous amplification of multiple amplicon targets in a polymerase chain reaction (PCR) with universal indexing. Finally, the technology uses a single set of common indexing oligonucleotides that can be used with any set of amplicons.

Additional information

Patent number and inventor

10,550,427

Jolene Bowers, Brandon Kitchel, Elizabeth Driebe, Duncan MacCannell, Chandler Roe, Darrin Lemmer, Tom de Man, J. Kamile Rasheed, David Engelthaler, Paul Keim, and Brandi Limbago.

Potential applications

Clinical and forensic applications for sample analysis.

Benefits and advantages

The UMINGAS technology addresses the need for high-throughput amplicon sequencing for genotyping, identifying the genetic variations. In turn, this can help minimize barcoding, when hundreds or thousands of samples are involved. Additionally, the technology can be configured to enable relatively inexpensive and efficient preparation of samples for use with downstream sequencing platforms. Furthermore, the technology is comprised of universal tail sequences that can enable the production of sequencing platform-ready amplicons in a multiplex fashion.

Case number and licensing status

2014-025

This invention is available for licensing.